Phosphotyrosyl-protein phosphatase of TCRC-2 cells.
نویسندگان
چکیده
منابع مشابه
Human prostatic acid phosphatase has phosphotyrosyl protein phosphatase activity.
The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase...
متن کاملPhosphotyrosyl-specific protein phosphatase activity of a bovine skeletal acid phosphatase isoenzyme. Comparison with the phosphotyrosyl protein phosphatase activity of skeletal alkaline phosphatase.
A partially purified bovine cortical bone acid phosphatase, which shared similar characteristics with a class of acid phosphatase known as tartrate-resistant acid phosphatase, was found to dephosphorylate phosphotyrosine and phosphotyrosyl proteins, with little activity toward other phosphoamino acids or phosphoseryl histones. The pH optimum was about 5.5 with p-nitrophenyl phosphate as substra...
متن کاملCharacterization of the phosphotyrosyl protein phosphatase activity of calmodulin-dependent protein phosphatase.
Calmodulin-dependent protein phosphatase from bovine brain and heart was assayed for phosphotyrosine and phosphoserine phosphatase activity using several substrates: 1) smooth muscle myosin light chain (LC20) phosphorylated on tyrosine or serine residues, 2) angiotensin I phosphorylated on tyrosine, and 3) synthetic phosphotyrosine- or phosphoserine-containing peptides with amino acid sequences...
متن کاملConversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase.
By use of the autophosphorylated epidermal-growth-factor receptor and the synthetic peptide RRLIE-DAEY(P)AARG, representing an autophosphorylation site of the transforming protein of Rous-sarcoma virus, it is demonstrated that the phosphotyrosyl phosphatase activity of the polycation-stimulated phosphatases is substantially increased by an enzyme-directed effect of ATP or PPi. Concomitant with ...
متن کاملPhosphotyrosyl protein phosphatases.
Enzyme-catalysed reversible protein phosphorylation is an important cellular regulatory mechanism (Nimmo & Cohen, 1977; Krebs & Beavo, 1979). Regulatory protein phosphorylation occurs most frequently on seryl and threonyl residues (Taborsky, 1974), and less frequently, on tyrosyl residues (Hunter, 1982). Protein phosphotyrosine [Tyr(P)] normally accounts for only 0.01-0.050o of the total protei...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34374-6